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Addgene inc grna lentiviral library

A. V-abl B cells expressing Flag-tagged Cas9 were infected with a BFP-expressing <t>lentiviral</t> <t>gRNA</t> library. BFP+ cells were sorted and treated with doxycycline for five days to induce Cas9 expression. Indicated inhibitors treatment was performed at IC90 for six days. Then, DNA was collected from the cells for sequencing and analysis using the MAGeCK pipeline. B. Scatter plot of total CRISPR genes Z-scores for Niraparib and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. C. Scatter plot of total CRISPR genes Z-scores for PDD0017273 and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. D. Summary table of Pearson R coefficient for linear regression between cell lines sensitivity to PDD, olaparib, nirabarib and talazoparib. Depmap sensitivity score (PRISM repurposing data) of 536 cell lines to the indicated compound. Simple linear regression and Pearson correlation test were performed to determine R. Colored scaled is represented on the right and R coefficients are written in white. E. Heatmap representing z-score (color range) and False Discovery Rate FDR (size) for the genes targeting indicated in rows, in cells challenged with inhibitor indicated above, in columns. Gene implicated in homologous recombination are displayed. F. CRISPR screen z-scores ranking of co-essential genes with PDD00017273 treatment. SSBR related genes are shown in red, ADP-ribosylation related genes are shown in dark blue, HR related genes are shown in light grey.

Grna Lentiviral Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/Mouse+Improved+Genome-wide+Knockout+CRISPR+Library+v2+(Pooled+Library+%2367988)/bio_rxiv__64898__2026__03__18__712393-209-8-11
Average 94 stars, based on 48 article reviews

grna lentiviral library - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity"

Article Title: PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity

Journal: bioRxiv

doi: 10.64898/2026.03.18.712393

A. V-abl B cells expressing Flag-tagged Cas9 were infected with a BFP-expressing lentiviral gRNA library. BFP+ cells were sorted and treated with doxycycline for five days to induce Cas9 expression. Indicated inhibitors treatment was performed at IC90 for six days. Then, DNA was collected from the cells for sequencing and analysis using the MAGeCK pipeline. B. Scatter plot of total CRISPR genes Z-scores for Niraparib and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. C. Scatter plot of total CRISPR genes Z-scores for PDD0017273 and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. D. Summary table of Pearson R coefficient for linear regression between cell lines sensitivity to PDD, olaparib, nirabarib and talazoparib. Depmap sensitivity score (PRISM repurposing data) of 536 cell lines to the indicated compound. Simple linear regression and Pearson correlation test were performed to determine R. Colored scaled is represented on the right and R coefficients are written in white. E. Heatmap representing z-score (color range) and False Discovery Rate FDR (size) for the genes targeting indicated in rows, in cells challenged with inhibitor indicated above, in columns. Gene implicated in homologous recombination are displayed. F. CRISPR screen z-scores ranking of co-essential genes with PDD00017273 treatment. SSBR related genes are shown in red, ADP-ribosylation related genes are shown in dark blue, HR related genes are shown in light grey.

Figure Legend Snippet: A. V-abl B cells expressing Flag-tagged Cas9 were infected with a BFP-expressing lentiviral gRNA library. BFP+ cells were sorted and treated with doxycycline for five days to induce Cas9 expression. Indicated inhibitors treatment was performed at IC90 for six days. Then, DNA was collected from the cells for sequencing and analysis using the MAGeCK pipeline. B. Scatter plot of total CRISPR genes Z-scores for Niraparib and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. C. Scatter plot of total CRISPR genes Z-scores for PDD0017273 and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. D. Summary table of Pearson R coefficient for linear regression between cell lines sensitivity to PDD, olaparib, nirabarib and talazoparib. Depmap sensitivity score (PRISM repurposing data) of 536 cell lines to the indicated compound. Simple linear regression and Pearson correlation test were performed to determine R. Colored scaled is represented on the right and R coefficients are written in white. E. Heatmap representing z-score (color range) and False Discovery Rate FDR (size) for the genes targeting indicated in rows, in cells challenged with inhibitor indicated above, in columns. Gene implicated in homologous recombination are displayed. F. CRISPR screen z-scores ranking of co-essential genes with PDD00017273 treatment. SSBR related genes are shown in red, ADP-ribosylation related genes are shown in dark blue, HR related genes are shown in light grey.

Techniques Used: Expressing, Infection, Sequencing, CRISPR, Homologous Recombination

Transduction:

Article Title: Senataxin and DNA-PKcs redundantly promote non-homologous end joining repair of DNA double strand breaks during V(D)J recombination.
Article Snippet: .. For each screen, ~180 × 106 cells were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting 18,424 mouse genes (Addgene, #67988) at a 40 to 50% transduction efficiency, as determined by the percentage of blue fluorescent protein (BFP)–positive cells after transduction. ..

Article Title: Senataxin and DNA-PKcs redundantly promote non-homologous end joining repair of DNA double strand breaks during V(D)J recombination
Article Snippet: .. For each screen, ~180 × 10 6 cells were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting 18,424 mouse genes (Addgene, #67988) at a 40 to 50% transduction efficiency, as determined by the percentage of blue fluorescent protein (BFP)–positive cells after transduction. ..

Article Title: Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progression
Article Snippet: .. More than 140 million wild-type Abl pre-B cells carrying inducible Cas9 transgene were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting over 18,000 mouse genes (Addgene, 67988) by spin-infection as described above. .. 3 days post-infection, cells transduced with gRNAs were sorted on a BD FACSAria II Cell Sorter based on BFP expression.

Article Title: Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progression
Article Snippet: .. More than 140 million wild- type Abl pre- B cells carrying inducible Cas9 transgene were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting over 18,000 mouse genes (Addgene, 67988) by spin- infection as described above. .. 3 days post- infection, cells transduced with gRNAs were sorted on a BD FACSAria II Cell Sorter based on BFP expression.

Article Title: Senataxin and DNA-PKcs Redundantly Promote Non-Homologous End Joining Repair of DNA Double Strand Breaks During V(D)J Recombination
Article Snippet: .. For each screen, ∼180×10 6 cells were transduced with a lentiviral gRNA library containing 90230 gRNAs targeting 18424 mouse genes (Addgene #67988) at a 40-50% transduction efficiency, as determined by the percentage of BFP+ cells after transduction ( ). ..

CRISPR:

Article Title: Zinc transporter 1 functions in copper uptake and cuproptosis.
Article Snippet: In brief Li et al. discovered that Zn transporter ZnT1 also imports copper (Cu) to cells, an activity required for copper-induced cell death, cuproptosis.. Structural and functional analysis revealed that Cu and Zn share the same primary binding site in ZnT1, with Zn competing for ZnT1mediated Cu uptake.. Physiologically, ZnT1 contributes to the maintenance of Lgr5+ intestinal stem cells and may be a potential target for the Zn-mediated Wilson’s disease treatment.

Infection:

Article Title: Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progression
Article Snippet: .. More than 140 million wild- type Abl pre- B cells carrying inducible Cas9 transgene were transduced with a lentiviral gRNA library containing 90,230 gRNAs targeting over 18,000 mouse genes (Addgene, 67988) by spin- infection as described above. .. 3 days post- infection, cells transduced with gRNAs were sorted on a BD FACSAria II Cell Sorter based on BFP expression.

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